A scientific analysis group from the Division of Paediatrics and Adolescent Medication and the Division of Obstetrics and Gynaecology, College of Scientific Medication, LKS College of Medication, The College of Hong Kong (HKUMed) leads the invention of making use of amniotic fluid cells obtained throughout 16-24 weeks of being pregnant as a novel pattern sort for RNA-sequencing in prenatal prognosis to assist extra households with tailor-made scientific administration. It’s the first proof-of-concept examine to show the potential scientific utility of amniotic fluid cells RNA-sequencing within the literature. The bottom-breaking findings have been printed within the tutorial journal, npj Genomic Medication.
Uncommon ailments are often genetic in origin. Though individually uncommon, collectively uncommon ailments had been discovered to be 1 in 67 within the Hong Kong inhabitants, primarily based on the group’s earlier examine.The identification of genetic trigger in uncommon ailments can present correct counselling for higher scientific administration and future being pregnant planning, which is crucial to help the sufferers. Present applied sciences for prenatal prognosis are largely DNA-based, with a big proportion (60-70%) stays undiagnosed, resulting in scientific uncertainty and parental anxiousness. Not too long ago, RNA-sequencing has been discovered to extend diagnostic yield by 10% to 36%, nevertheless, none of those research centered on prenatal prognosis. As well as, despite the supply of well-established massive database cataloging the gene expression profile of various tissues for grownup, comparable publicly-available dataset for amniotic fluid cells reflecting the embryological and fetal stage is missing. Additional examine and analysis on RNA-sequencing in prenatal setting is thus essential.
The analysis group demonstrated the potential scientific utility of amniotic fluid cells RNA-sequencing. A baseline for gene expression profile of amniotic fluid cells is established by performing RNA-sequencing on over 50 amniotic fluid samples. Institution of gene expression profile is an important step in making use of RNA-sequencing to the present chosen scientific prognosis workflow. They discovered that the variety of well-expressed genes in amniotic fluid cells was similar to different clinically accessible tissues generally used for genetic prognosis throughout completely different illness classes. The analysis group additionally in contrast RNA-sequencing information of 4 affected fetuses with structural congenital anomalies with the established baseline to detect potential outliers. In collaboration with the Technical College of Munich in Germany, a bioinformatics pipeline was tailored to boost the detection of outliers for subsequent evaluation. Additional in-depth curation confirmed that outliers will be recognized in genes related to the corresponding structural congenital anomalies in all 4 affected fetuses. Figuring out the outliers present extra proof on the RNA degree to assist prognosis.
The findings of this examine have important implications in fixing undiagnosed uncommon ailments in Hong Kong. It’s the first time that amniotic fluid cells RNA-sequencing is reported to supply potential scientific utility in prenatal prognosis in literature. With the identification of the genetic trigger, precision drugs akin to tailor-made scientific administration and preimplantation genetic prognosis for households with household historical past is feasible.
This examine was chosen because the ‘Reviewers’ Alternative Summary’ (high 10% of poster summary) on the American Society of Human Genetics (ASHG) Annual Assembly 2022, one of many largest conferences of its variety, during which scientists from around the globe are chosen to current their cutting-edge analysis findings collaboratively . ‘It’s an honour for HKUMed poster to be chosen and scored as the highest 10% of poster abstracts on this worldwide convention. RNA-sequencing has nice potential in fixing unexplained genetic problems. We are going to proceed to strengthen ties and collaborations with establishments in Hong Kong and overseas, contribute to worldwide scientific analysis and produce advantages again to our native sufferers,’ stated Dr Brian Chung Hon-yin, Scientific Geneticist, Scientific Affiliate Professor, Division of Paediatrics and Adolescent Medication, College of Scientific Medication, HKUMed.
Dr Anita Kan Sik-yau, Honorary Scientific Affiliate Professor, Division of Obstetrics and Gynaecology, College of Scientific Medication, HKUMed cum Deputy Laboratory Director of the Prenatal Diagnostic Laboratory at Tsan Yuk Hospital added, ‘On the memorable event of Tsan Yuk Hospital (TYH)’s a hundredth Anniversary, this examine demonstrated TYH’s distinctive service for Hong Kong over the previous century, and steady effort to contribute to all these in want, alongside revolutionary analysis.’
This analysis was led by Dr Brian Chung Hon-yin, Scientific Affiliate Professor, Division of Paediatrics and Adolescent Medication, College of Scientific Medication, HKUMed; Dr Anita Kan Sik-yau, Honorary Scientific Affiliate Professor, Division of Obstetrics and Gynaecology, College of Scientific Medication, HKUMed. Mianne Lee, MPhil candidate, and Dr Anna Kwong Ka-yee, Assistant Analysis Officer, Division of Paediatrics and Adolescent Medication, College of Scientific Medication, HKUMed are the primary authors.
Different contributors included Dr Christopher Mak Chun-yu, Martin Chui Man-chun, Jeffrey Chau Fong-ting, Division of Paediatrics and Adolescent Medication, College of Scientific Medication, HKUMed; Dr Kelvin Chan Yuen-kwong, Dr Sandy Au Leung-kuen and Lo Hei-man, Division of Obstetrics and Gynaecology, College of Scientific Medication, HKUMed. Worldwide collaborators had been Professor Julien Gagneur and Dr Vicente A Yépez from Division of Informatics, Technical College of Munich, Garching, Germany.
This examine was supported by The Society for the Reduction of Disabled Kids, Well being and Medical Analysis Fund of the Well being Bureau, the Authorities of the Hong Kong Particular Administrative Area and the Mr and Mrs Edward CM Wong Basis.